What Is Heat Inactivated FBS?
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- Olivia Grande
- March 3, 2023
The FBS word is an acronym for fetal bovine serum. Since most of us wouldn’t be familiar with what is heat inactivated FBS, firstly they need to understand that fetal bovine serum heat inactivated is an important serum that is added in cell culture medium. It is very beneficial to balance the health of cells and to promote their expansion in your body. The FBS consists of many healthy and beneficial factors that are very good for expanding cell growth.
The serum consists of numerous elements like amino acids, lipids, sugars, etc and all of these are very critical to promote the overall cell culture. FBS is basically a component found in the blood and can be collected by the removal of cellular components from the person. The FBS is mixed with many different components and molecules and that’s the main reason why it is so important for promoting the cellular growth and increasing the expansion.
The FBS is created under strict guidance to ensure maximum quality and efficient result of the product and that’s the reason why we say buy heat inactivated FBS from universal Bio Products. Due to strict quality control, there are no chances of getting any side effect from using our serum as we give special importance to the health of our customers.
What Does Heat Inactivated FBS Mean?
The process of heat activation is very well known to use the fetal bovine serum. The serum is carried throughout the procedure to make it usable. The serum is heated at a temperature of 56 to 60 degrees Celsius to make it inactivated completely. It is heated for nearly thirty minutes at the same temperature under strict control conditions. The reason behind inactivating the serum is to achieve the objective that is to eliminate any kind of activity from complement within the serum while ensuring that the growth promoting characteristics of the serum doesn’t get affected at all.
Although the elimination of complement activity isn’t needed for most of the cellular cultures, but it might be necessary for cell cultures that are sensitive and can get affected by the activity of complement. The negative effect of the heat inactivation is that it can possibly decrease the growth performance property of the serum. Due to this specific reason, the procedure of heat inactivation should only be carried out if it is necessary for a specific cell culture to ensure growth and expansion. The researchers and the ones who prepare the serum should be aware that what cell culture requires heat inactivation and should carry it accordingly for efficient growth of cells.
If the condition tells that heat activation is required then it should be carried out through the heat while assuring that it is carefully carried out to eliminate any chances of increased formation of crystalline and flocculent precipitates, gelling of proteins within the serum and high loss of growth performance. The serum can be damaged severely if it is passed through a temperature that wasn’t required or if it is heated for a long period of time. The process of heat inactivation should be carried out very carefully so that the user can use it and gain maximum results from it.
Collection, processing, and testing of FBS
Collection: The product is produced and collected in such a way that it meets all the demands set by the USDA and the product can be easily relocated to its date of production. The manufacturing of the FBS sticks to closed system collection, easily processing in high and good pressures, HEPA cleaned rooms and labs, and filtration system of 0.1um to make sure that all the contaminants are eliminated. Due to strict control and guidelines provided by us, the FBS that we produce from collection to processing ensures that a stable, continuous performing and traceable supply of serum is supplied throughout the globe to ensure a healthy life for everyone. Our quality isn’t compromised at all during the process of collection and processing
Virus testing: The FBS that is produced is tested for severe kinds of viruses like Bovine Viral Diarrhea Virus (BVDV), Bluetongue virus (BTV), Bovine respiratory syncytial Virus (BRSV), Infectious bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 virus, Bovine Parvovirus (BPV), Bovine Adenovirus type 3 (BAV-3), Bovine Adenovirus type-5 (BAV-5), Reo virus, and Rabies virus.
Biochemical testing: the FBS is undergone biochemical profiling and testing and should ensure the presence of total protein, albumin, globulin, total bilirubin, iron, glucose, triglycerides, sodium, potassium, and cholesterol. Every serum might have different levels of the biochemical so make sure to read the inert before buying it or try to contact the doctor before ordering one.
Chemical analysis: every single one of the fetal bovine serum (FBS) is tested for osmolality, endotoxin by LAL method, pH level, Hemoglobin, and microbiological contaminations. These microbiological contaminations are suggested or recommended by the U.S pharmacopeia to determine what levels of these chemicals should be present in the serum to make it available for the customers or patients to use.